Techniques

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2-photon Calcium Imaging

We used CRISPR to express the calcium indicator gCaMP6 in Aedes aegypti mosquitoes, allowing us to image neural activity in the brain while presenting sensory stimuli.

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Immunohistochemistry

We apply fluorescently tagged antibodies against neuromodulators and other proteins involved in learning and memory to visualize their locations in the brain. 

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3-Dimensional Reconstruction

Reconstruction software allows us to convert image stacks of brain structures into 3D models used to understand spatial relationships and volumes of neural tissues.

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Behavior

We assay animal behaviors including movement of limbs, head, or wings and performance in mazes, then analyze differences in these behaviors due to learning or induced physiological changes.

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Histology

We use chemical stains and dyes to visualize neural structures at different microscopic scales:

     • Gross anatomy

     • Cell morphology

     • Sub-cellular structures